ASCP ASCP Certifications ASCP-MLT Questions & Answers

• Question 151:

  Isotonic or normal saline is a 0.85 % solution of sodium chloride in water.

  The concentration of sodium chloride in an isotonic solution is :

  A. 8.5 %

  B. 0.85 %

  C. 0.08 %

  D. 1 molar

  Correct Answer: B

• Question 152:

  Overcentrifugation may cause either a false negative result (if too much agitation is required for resuspension), or a false positive, (if centrifuged clumps cannot be completely dispersed). High concentration of IgG paraprotein, and failure to adequately wash cells can leave unbound IgG which will neutralize antiglobulin reagent. Delay of addition of antiglobulin reagent may allow previously bound IgG antibody to dissociate from red cells.

  Blood bank

  Which of the following might cause a false positive indirect antiglobulin test:

  A. Failure to adequately wash cells

  B. Delay of addition of antiglobulin reagent.

  C. Overcentrifugation

  D. High concentration of IgG paraprotein in a patient's serum

  Correct Answer: C

• Question 153:

  A. Lavender

  B. Light blue

  C. Gray

  D. Green

  Correct Answer: D

• Question 154:

  Protein in urine can be confirmed using sulfosalicylic acid (SSA) precipitation. The SSA reagent is added to a small volume of urine. Acidification causes precipitation of protein in the sample, which is subjectively graded as trace, 1+, 2+, 3+ or 4+. SSA reaction will detect albumin, globulins, and Bence-Jones proteins.

  Which of the following would be the most appropriate method to confirm a positive protein from a urine dipstick:

  A. Immunoelectropheresis

  B. Heat precipitation

  C. Sulfosalicylic acid precipitation

  D. Protein electrophoresis

  Correct Answer: C

• Question 155:

  All of the following tubes contain separation gel except: Question options:

  A. gold

  B. green

  C. light green or green-gray

  D. red/gray

  Correct Answer: B

• Question 156:

  Gluconeogenesis is the formation of glucose from noncarbohydrates when carbohydrate intake is absent, a fasting state. The hormone cortisol along with glucagon and epinephrine all stimulate this metabolic pathway. Insulin; however, inhibits this pathway and is therefore the correct answer.

  Which of the following hormones inhibits gluconeogenesis, the formation of glucose from noncarbohydrate sources such as amino acids, glycerol, and fatty acids?

  A. Insulin

  B. Epinephrine

  C. Cortisol

  D. Glucagon

  Correct Answer: A

• Question 157:

  Hemoglobin electrophoresis uses an electric field to separate hemoglobin molecules based on differences in net electrical charge. The rate of electrophoretic migration is also dependent on the ionic radius of the molecule, the viscosity of the solution through which it is migrating, the electrical field strength, temperature, and the type of supporting medium used.

  Electrophoretic separation of hemoglobin fundamentally relies on:

  A. Size differences of molecules

  B. Net charge differences of molecules

  C. Concentration differences of molecules

  D. Shape variations of molecules

  Correct Answer: B

• Question 158:

  Which specimen should be collected last? Question options:

  A. Specimen requiring special handling

  B. Clotted specimen

  C. Fasting specimen

  D. STAT specimen

  Correct Answer: D

• Question 159:

  First, determine the number of WBC's from the hemocytometer as follows:

  WBC count = (dilution ratio x # of cells counted x 10) / (# mm2 area counted) Then: WBC count = (20 x 100 x 10) / (8) = 2500 WBC/mm3 (or 2500 WBC/uL or 2.5 x 103 WBC/uL)

  Next, to find the WBC count per liter, multiply the WBC count/uL by the number of uL/L (there are 106 uL/L)

  So: (2.5 x 103 WBC/uL) x (106 uL/L) = 2.5 x 109 WBC/L

  Hematology

  A 1:20 dilution is made for a manual WBC count. The four corner squares on both sides of a hemocytometer are counted. A TOTAL of 100 cells are counted in that area. What is the white blood cell count in terms of a liter (? x 10^9/L)?

  A. 2.5

  B. 25

  C. 250

  D. 2500

  E. 25000

  Correct Answer: A

• Question 160:

  L/S ratio <2.0 indicates an increased risk of respiratory distress syndrome (RDS) at delivery. L/S ratio <1.5 indicates a very high risk of developing RDS.

  Which of the following statements regarding the L/S ratio is TRUE?

  The correct answer is highlighted below

  A ratio of 2:1 or greater usually indicates adequate pulmonary surfactant to prevent respiratory distress syndrome (RDS)

  A ratio of 1.5:1 indicates fetal lung maturity in pregnancies associated with diabetes mellitus.

  Sphingomyelin levels increase during the 3rd trimester causing the L/S ratio to fall slightly during the last two weeks of gestation.

  A phosphatidylglycerol (PG) spot indicates the presence of meconium in the amniotic fluid Lecithin is in direct ratio with sphingomyelin

  Heparin contamination is characterized by an elevation in the aPTT test and can also cause an increased PT test as well. Reptilase time tests are used to elimate the effects of heparin contamination as the reagents and method are resistant

  to the effects of antithrombin III, unlike the PT and aPTT tests. Therefore, it would be expected that a patient sample containing pre-analytical heparin contamination will show an increased aPTT (and sometimes PT as well) while showing a

  normal reptilase time.

  A specimen drawn from an indwelling catheter that was contaminated by heparin would be indicated by:

  A. normal PT and aPTT results

  B. abnormal PT and normal PTT results

  C. prolonged aPTT and prolonged reptilase time test

  D. prolonged aPTT and normal reptilase time test

  Correct Answer: D