ASCP ASCP Certifications ASCP-MLT Questions & Answers

• Question 341:

  To make a 1 : 10 dilution, 270 μL of diluent should be added to 30 μL of sample, .

  A spinal fluid that is slightly hazy is briefly examined microscopically. The technologist performing the count decides to make a 1:10 dilution using 30 μL of sample. What volume of diluent should be used?

  A. 70 μL

  B. 270 μL

  C. 300 μL

  Correct Answer: B

• Question 342:

  An ultrarapid metabolizer (UM) would require a higher dose of a drug than an EM (a person with normal enzyme activity) since the UM eliminates the drug more quickly.

  A CYP2D6 ultrarapid metabolizer (UM) would require ___________ dose of an active drug (non-prodrug) that is metabolized by CYP2D6 than a CYP2D6 extensive metabolizer (EM).

  A. a higher

  B. a lower

  C. the same

  Correct Answer: A

• Question 343:

  Though automated extraction machines have many benefits over manual methods the costs are high and generally require a high throughput of samples in order to justify the costs.

  Automated extraction has many benefits over the traditional manual methods. The most important benefit is that the nucleic acid isolated is constantly consistent. There is a reduced amount of manipulation with dramatically decreases the chance of cross contamination. Also, automated extraction machines are considered moderate complexity and can be performed by a wider variety of laboratory professionals. All of the following are considered benefits of automated isolation and extraction equipment EXCEPT:

  A. Automated isolation equipment is cheaper and more practical

  B. Automated isolation equipment requires less manipulation

  C. Automated isolation equipment is considered moderate complexity

  D. Automated isolation equipment provides consistent results

  Correct Answer: A

• Question 344:

  Drawing a lavendar stopper tube before an SST tube can cause a falsely: Question options:

  A. decreased glucose value

  B. decreased calcium value

  C. increased calcium value

  D. increased glucose value

  Correct Answer: B

• Question 345:

  The steps in the PCR process are:

  1.

  Denaturation (Turning double stranded DNA into single strands.)

  2.

  Annealing/Hybrization (Attachment of primers to the single DNA strands.)

  3.

  Extension (Creating the complementary strand to produce new double stranded DNA.) What is the first step of the PCR reaction?

  A. Hybridization

  B. Extension

  C. Annealing

  D. Denaturation

  Correct Answer: D

• Question 346:

  The role of provider-performed microscopy (PPM) is the

  A. Process of performing laboratory testing at the bedside of the patient and a means of decentralizing some of the laboratory testing.

  B. Specific microscopic tests (wet mounts) performed by a physician for his or her own patients.

  C. Means by which quality control between laboratories is maintained.

  D. both moderately and highly complex

  Correct Answer: B

• Question 347:

  Autologous units must be drawn before they are needed, and must be readily available, therefore are generally not of use in emergencies. All of the following are benefits of autologous donation except:

  A. Reduces exposure to infectious agents

  B. Are always on hand in case of an unexpected emergency

  C. Reduces demand for homologous blood

  D. Eliminates sensitization to cellular blood components

  Correct Answer: B

• Question 348:

  The Needlestick Safety and Prevention Act requires employers to:

  A. test MLTs annually for hepatitis B and HIV

  B. involve MLTs in the selection of safety devices

  C. provide MLTs with plastic capillary tubes

  D. allow MLTs to work flexible schedules

  Correct Answer: B

• Question 349:

  A positive DAT is consistent with a diagnosis of hemolytic disease of the newborn. A hemoglobin performed on the cord blood serves as an indicator of the severity of the disease. In order to detect the presence of antibodies fixed on the RBC's of newborns one should:

  A. Perform a direct antiglobulin test (DAT) on the cord blood

  B. Perform an indirect antiglobulin test (IAT) on the cord blood

  C. Test the baby's cells with typing antiserum

  D. Look for agglutination in a mixture of mother's serum and baby's cells

  Correct Answer: A

• Question 350:

  In an alkaline medium, potassium ferricyanide oxidizes hemoglobin to methemoglobin. Further reaction with potassium cyanide produces cyanmethemoglobin which has a maximum absorbance at 540 nm. Color intensity is proportional to

  total hemoglobin concentration, which is how the hemoglobin levels are measured.

  Hematology

  In the hemoglobin methodology using potassium ferricyanide the following measurable reaction occurs:

  A. carboxhemoglobin is formed

  B. iron remains in the ferrous state

  C. ferrous iron is oxidized to ferric iron to form methemoglobin

  D. acid hematin is formed

  Correct Answer: C